The second part of the paper analyses the method of assessing impact of motorways on arable lands, proposed in the first part. The method can be used to determine all the losses resulting from different directions of this impact. The losses are determined in an analysis of variations in land use, soil quality class, layout of access roads leading to lands situated along the axis of a planned motorway. The method is especially useful at the initial planning of a motorway or at evaluation of its routes’ variants, because it is simple and helps to determine a negative impact of a motorway on arable lands with adequate accuracy. The impact of a motorway on arable lands is exemplified by a Tarnów – Rzeszów section of an existing A4 motorway. The section is 7.136 km long and it runs through Borowa, Wewiórka and Góra Motyczna villages. The outcomes were compared with an alternative variant of the motorway’s route in this section.